A STUDY OF PRE-ANALYTICAL AND STORAGE-RELATED HEMATOLOGICAL VARIATIONS IN PACKED RED BLOOD CELLS IN A TERTIARY CARE BLOOD BANK

Background:Packed red blood cells (PRBCs) represent the most frequently transfused blood component worldwide. During storage, PRBCs undergo progressive biochemical and morphological alterations collectively termed the "storage lesion," which may compromise transfusion efficacy and patient outcomes. Pre-analytical variables including collection techniques and processing times further influence product quality.Methods:This prospective observational study analyzed 120 PRBC units collected from voluntary blood donors. Pre-analytical variablesincluding ambient temperature exposure and processing time were documented. Hematological parameters including hemoglobin, hematocrit, mean corpuscular volume (MCV), red blood cell count, potassium levels, pH, lactate concentrations, and percentage hemolysis were measured at days 0, 7, 14, 21, 28, and 35 of storage.Results:Significant progressive changes were observed throughout the storage period. Hemoglobin decreased from 18.42 ± 1.28 g/dL to 17.15 ± 1.34 g/dL (p