Understanding the Pharmacokinetic Effect of Aspartame and Nateglinide Against Hydrolase: An Insilico Study

This study is performed to understand the pharmacokinetic effect of aspartame over nateglinide by an insilico approach. Virtual screening was performed for the analogues of aspartame and nateglinide against the hydrolase enzyme (3BER) involved in the metabolism the drugs using AUTO DOCK/GOLD. Molecular docking was used to evaluate a competitive interaction study of analogues. The results reviled that the amino acids THR75, THR70 and TRP44 of the active site contributed for the hydrogen bond interaction with both analogous. Aspartame analogues m843_10 and nateglinide analogues m49_2 showed the highest binding energy generating the highest F-Score of -73.94 kcal/moland -54.59kcal/mol respectively. It can be concluded that interaction profile providea roadmap that analogues interact with the same binding site and act as a competitive inhibitor leading to less hypoglycemic effect and thus it’s hypothesis that nateglinide should not be taken along with the artificial sweetener aspartame resulting in leading to low hypoglycemic activity.