Seroprevalence of Toxoplasma gondii Infection and Its Association with Gender and Age among Patients Attending Al-Bayda Medical Center, Libya

Toxoplasma gondii is a globally prevalent intracellular protozoan parasite and a significant public health concern due to its asymptomatic nature, lifelong persistence, and potential to cause severe disease in vulnerable populations. This hospital-based cross-sectional study aimed to determine the seroprevalence of T. gondii infection and its association with age and gender among patients attending Al-Bayda Medical Center, Libya, in 2025. A total of 124 patients (94 females, 30 males) aged 0.05), whereas age was significantly associated with IgG positivity (p < 0.05), with older individuals more likely to exhibit past infection. The high proportion of seronegative individuals highlights a large at-risk population, especially among women of childbearing age. These findings provide baseline epidemiological data for T. gondii infection in eastern Libya and underscore the importance of preventive measures, including public awareness campaigns and routine serological screening, to reduce the risk of primary infection and potential congenital toxoplasmosis. The study emphasizes age as a key determinant of exposure, while gender appears not to influence seropositivity in this population.