Effect of Yangyin Yiqi Formula （养阴益气方） on MUC1-mediated epithelial mesenchymal transition in pulmonary fibrosis

Lishizhen Medicine and Materia Medica Research 2026 Open access

Read full text
Authors: Lihong Meng, Hui Li, Xiaomei ZHANG, Mengzhen WANG, Min Wu, Bobo ZHOU, Fang Shang, Yanbo ZHANG, Xiaoming XUE
DOI: 10.70976/j.1008-0805.szgygy-2026-01-0106
Cited by: 0

Abstract

目的 探讨养阴益气方通过调控粘蛋白1（MUC1）介导的上皮间质转化（EMT）抑制肺纤维化进展的机制。 方法 利用siRNA-MUC1转染A549细胞，以转化生长因子β1（TGF-β1）刺激转染细胞，Western blot和RT-PCR法检测EMT相关指标表达。以TGF-β1刺激A549细胞建立肺纤维化细胞模型，将细胞分为对照组、模型组、空白血清组、养阴益气方组（YYYQ）、转染＋养阴益气组（siRNA-MUC1＋YYYQ），予相应药物进行干预，再以Western blot及 RT-PCR法检测指标变化。 结果 在A549细胞中，转染 siRNA-MUC1 后，转染细胞中α-SMA、Vimentin水平下降，E-cadherin表达升高。以养阴益气含药血清干预细胞后，与对照组相比，模型组细胞中MUC1、α-SMA、Vimentin、Snail、SLUG、COL-I 表达显著升高，E-cadherin表达下调（P

Fields of Study & Subjects

Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis Systemic Sclerosis and Related Diseases Pulmonary Hypertension Research and Treatments

Publication details

Journal / source
Lishizhen Medicine and Materia Medica Research
Volume
37
Issue
1
Pages
40–45
Publication date
1 January 2026
Language
EN