Effect of Tongxie Yaofang （痛泻要方） on intestinal mucosal barrier function and gut microbiota in rats with diarrhea-predominant irritable bowel syndrome

Lishizhen Medicine and Materia Medica Research 2026 Open access

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Authors: Xiangyu Xie, Hongbin LI, Xiuli GONG, Wei Ke, Qin LU, Zhifeng HUANG, Hongmei TANG, He ZHU
DOI: 10.70976/j.1008-0805.szgygy-2026-0201
Cited by: 0

Abstract

目的 探讨痛泻要方对腹泻型肠易激综合征（IBS-D）大鼠肠黏膜屏障功能和肠道菌群的影响。 方法 将30只SPF级SD大鼠随机分为空白组、模型组、痛泻要方（TXYF）组（6.75 g生药/kg），每组10只。采用三因素法（母婴分离+醋酸刺激+束缚），复制IBS-D大鼠模型，造模结束后，给予TXYF连续灌胃14 d，采用腹部回缩反射（AWR）评价内脏高敏状态，HE染色观察大鼠结肠组织情况，采用透射电镜（TEM）观察结肠黏膜超微结构，采用ELISA法检测结肠组织中黏膜损伤相关蛋白二胺氧化酶（DAO）、D-乳酸（D-LA）水平，16S rRNA测序检测各组大鼠粪便中肠道菌群结构的变化情况。 结果 与空白组比较，模型组大鼠AWR评分升高，与模型组比较，TXYF组大鼠AWR评分降低（P

Fields of Study & Subjects

Gastrointestinal motility and disorders Gut microbiota and health Traditional Chinese Medicine Studies

Publication details

Journal / source
Lishizhen Medicine and Materia Medica Research
Volume
37
Issue
2
Pages
201–207
Publication date
1 January 2026
Language
EN