The effect of electroacupuncture on macrophage polarization in rats with primary dysmenorrhea based on the AMPK/Nrf2/HO-1 pathway

Lishizhen Medicine and Materia Medica Research 2026 Open access

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Authors: Yan ZI, Yuting PENG, Yanrong WEI, You Zhang, Yiwen TANG, Jing Zhou, Zenghui YUE
DOI: 10.70976/j.1008-0805.szgygy-2026-0227
Cited by: 0

Abstract

目的 探讨电针对原发性痛经（PDM）大鼠的改善作用及其对巨噬细胞极化的影响。 方法 将60只SD大鼠随机分为空白组、模型组、假电针组、电针组和西药组，每组12只。采用苯甲酸雌二醇+缩宫素构建周期性PDM大鼠模型；空白组和模型组仅捆绑固定，电针组电针双侧“三阴交”穴；假电针组于穴旁非经穴浅刺（不输出电流）；西药组给予布洛芬灌胃；干预均1次/d，连续5 d。观察扭体反应和子宫病理学变化；采用ELISA法检测血清前列腺素PGE2、PGF2α及炎症因子IL-6、TNF-α、iNOS、IL-10、Arg-1含量；采用免疫荧光法和Western blot法分别检测子宫巨噬细胞标志物iNOS、Arg-1及AMPK/Nrf2/HO-1通路蛋白表达。 结果 与空白组比较，模型组大鼠扭体反应加剧，子宫病理损伤严重，血清PGF2α、PGF2α/PGE2、TNF-α、IL-6、iNOS水平及子宫iNOS表达升高，而PGE2、IL-10、Arg-1水平、子宫Arg-1表达及p-AMPK/AMPK、Nrf2、HO-1蛋白表达均降低（P

Fields of Study & Subjects

Acupuncture Treatment Research Studies Pain Mechanisms and Treatments Traditional Chinese Medicine Studies

Publication details

Journal / source
Lishizhen Medicine and Materia Medica Research
Volume
37
Issue
2
Pages
378–384
Publication date
1 January 2026
Language
EN