Investigating the effect of Tongxie Yaofang Formula （痛泻要方） on the intestinal flora of IBS-D patients with liver depression of spleen deficiency based on 16S rDNA gene sequencing

Lishizhen Medicine and Materia Medica Research 2026

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Authors: Kuncheng ZHOU, Chengjiao YAO, Ye Li, Lihong LUO, Fengjiao Xie, Qin XIONG, Yang Yang, Junling Tang, Shiwen Zhou, Xiangxiong CHENG, Peimin Feng
DOI: 10.70976/j.1008-0805.szgygy-2026-0427
Cited by: 0

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Abstract

目的 探讨中国西南部人群中健康对照组与肝郁脾虚型IBS-D患者之间的肠道菌群及代谢物差异，初步评估痛泻要方对肝郁脾虚型IBS-D患者肠道菌群的影响。 方法 课题纳入了60例健康对照组与120例肝郁脾虚型IBS-D患者，并将IBS-D患者随机分为空白对照组、痛泻要方治疗组（n=60）。收集患者就诊前及就诊14天后的粪便样本以及健康志愿者体检当日的粪便样本，并通过16S rDNA基因测序技术检测健康对照组、痛泻要方治疗前后患者肠道菌群的变化。 结果 IBS-D和健康对照组的拟杆菌门/厚壁菌门（F/B）比率差异明显。经过痛泻要方治疗后，IBS-D的罗氏菌属（Roseburia）、罗姆布茨菌属（Roseburia spp.，Romboutsia timonensis）以及唾液链球菌（Streptococcus_salivarius）的丰度增高。KEGG富集结果显示：在IBS-D患者中，MAPK信号通路、自噬和T细胞受体信号通路的活性显著增强。 结论 健康对照组，肝郁脾虚型IBS-D患者和痛泻要方治疗后的IBS-D患者肠道菌群结构与功能均存在显著差异。痛泻要方可能通过增加罗氏菌属、罗姆布茨菌属、唾液链球菌等菌属的丰度，调节肠道菌群并对肝郁脾虚型IBS-D患者产生疗效。

Fields of Study & Subjects

Gut microbiota and health Gastrointestinal motility and disorders Traditional Chinese Medicine Studies

Publication details

Journal / source
Lishizhen Medicine and Materia Medica Research
Volume
37
Issue
4
Pages
781–789
Publication date
1 February 2026
Language
EN