Impact of base matrix types on the properties of affinity chromatography resins with ligands based on staphylococcal protein A B-domain multimers

Mendeleev Communications 2026

View at publisher
Authors: Milena V Baskova, Mikhail Nikolaevich Tereshin, Khristina P Telepenina, Natalia Sergeevna Shoshina, Alina Mikhailovna Komyakova, Barbara Zlatkovna Eletskaya, Maria Yanovna Berzina, Larisa Nikolaevna Ikryannikova, Vasily Nikolaevich Stepanenko, Tatiana Dmitrievna Melikhova
DOI: 10.71267/mencom.7973
Cited by: 1
References: 13

Abstract

New chromatography resins with ligands based on oligomers of the B-domain of Staphylococcus aureus protein A were developed and characterized. Resin performance, particularly dynamic binding capacity, improved as the ligand length increased from dimer to tetramer when immobilized on a polymethacrylate surface, whereas the opposite effect was observed when using cross-linked agarose as a matrix. It was found that particle size uniformity and pore volume can play a significant role and their careful control is necessary when selecting a matrix.

Fields of Study & Subjects

Protein purification and stability Biopolymer Synthesis and Applications Analytical Chemistry and Chromatography

Publication details

Journal / source
Mendeleev Communications
Publisher
OOO Zhurnal "Mendeleevskie Soobshcheniya"
Volume
36
Issue
4
Pages
404–406
Publication date
1 January 2026
Language
RU