Fermented Clostridium butyricum GKB7 Inhibits Osteoarthritis Induced by Anterior Cruciate Ligament Transection in a Preclinical Model

Aging-related osteoarthritis (OA) affects joints and causes functional impairment.Probiotics are recognized safe for consumption and numerous exhibit beneficial bioactivity for human health conditions.Clostridium butyricum, frequently found in the environment and reported to colonize the stomachs of adults and newborns, exhibits anti-inflammatory properties.This study investigated whether fermented Clostridium butyricum GKB7 is effective at preventing the advancement of OA.Fermented GKB7 reduces bone pain and the development of OA associated with anterior cruciate ligament transaction.Through the reduction of pro-inflammatory cytokines IL-1β and TNF-α, as well as the chondrolytic factors MMP-3, MMP-13, and ADAMTS5, fermented GKB7 inhibited the degradation of aggrecan and COL2A1.Bone loss and cartilage degradation were blocked as a result of this activity.According to our findings, fermented GKB7 enhances the prevention of OA development.