Development Of Molecular Resources for Genetic Diversity Studies in Sanguinaria Canadensis

Sanguinaria canadensis L. (Papaveraceae), is a monotypic species with a broad range. Though S. canadensis is relatively common throughout its range, it has been considered a species of concern due to its methods of reproduction and dispersal and is being exploited for medicinal purposes. This study was designed to elucidate the genetic diversity of selected populations of this species via the development of 11 microsatellite markers and the genotyping of 188 individuals from 6 populations located in North Carolina, Tennessee, and Virginia. Based on my findings, all 11 markers were polymorphic and successfully amplified for most individuals. These loci have moderate allelic diversity, ranging from 3 to 29 alleles per locus; moderate expected heterozygosity, ranging from 0.02 to 0.92; low observed heterozygosity, ranging from 0.01 to 0.40; and some differentiation, with the majority of molecular variance existing among individuals (90%). These results suggest a lack of gene flow between populations and possible restrictions to dispersal via pollination or seed movement. A cross-amplification experiment was also conducted in S. canadensis’ s sister taxon Eomecon, with amplification success in 82% of markers developed. The results of this study contribute to a more comprehensive understanding of the genetic diversity of S. canadensis populations.