[Effect of interleukin-33 on glucocorticoid-induced osteonecrosis of the femoral head in mice].

Objective: To elucidate the role of interleukin-33 (IL-33) in glucocorticoid-induced osteonecrosis of the femoral head (ONFH) in mice, with particular emphasis on its effects on bone remodeling, inflammatory regulation, and fibrosis. Methods: : Murine osteoblasts were divided into control group (DMEM+PBS), IL-33 group (DMEM+10 ng/mL IL-33), and IL-33+ST2L group (DMEM+10 ng/mL IL-33+1 μg/mL ST2L antibody). After corresponding treatment, cell proliferation was detected by EdU incorporation assay. Additional osteoblasts were subjected to osteogenic induction culture, and mineralization, and the expression of osteogenesis-related genes (Runx2, collagen type Ⅰ, OCN, and OPN) were assessed by using alkaline phosphatase (ALP) staining, Alizarin red staining, and qPCR, respectively. Results: >0.05). Conclusion: IL-33 exacerbates ONFH by impairing osteoblast viability and function and inhibiting bone regeneration. Targeting the IL-33/ST2L signaling axis may represent a promising novel therapeutic strategy for ONFH.