Reviewer #2 (Public review): Affinity-guided labeling reveals P2X7 nanoscale membrane redistribution during BV2 microglial activation

An affinity-guided chemical strategy enabling highly specific biotinylation of P2X7 receptors reveals, by super-resolution microscopy, how the nanoscale organization of endogenous P2X7 in BV2 microglial cells dynamically changes upon activation.