Reviewer #3 (Public review): Ribosomal RNA synthesis by RNA polymerase I is subject to premature termination of transcription

This manuscript characterizes a mutated clone of RNA polymerase I in yeast, referred to as SuperPol, to understand the mechanisms of RNA polymerase I elongation and termination.The authors present convincing evidence that demonstrates the existence of premature termination in Pol I transcription.Overall, the characterization of this RNA pol I offers important insights into the regulation of ribosomal RNA transcription and its potential application in cancer pharmacology.