Reviewer #1 (Public review): Arrayed single-gene perturbations identify drivers of human anterior neural tube closure

Roya E Huang; Giridhar M Anand; Heitor C Megale; Jason Chen; Chudi Abraham-Igwe; Sharad Ramanathan

doi:10.7554/elife.108224.2.sa1

Reviewer #1 (Public review): Arrayed single-gene perturbations identify drivers of human anterior neural tube closure

2026 Open access

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Authors: Roya E Huang, Giridhar M Anand, Heitor C Megale, Jason Chen, Chudi Abraham-Igwe, Sharad Ramanathan

DOI: 10.7554/elife.108224.2.sa1

Cited by: 0

Abstract

A method for arrayed CRISPRi screening in organoids enables the investigation of morphogenesis in human stem cell-derived tissues, revealing the roles of ZIC2, SOX11, and ZNF521 in neural tube closure.

Fields of Study & Subjects

Tissue Engineering and Regenerative Medicine

Publication details

Publication date: 27 April 2026