eLife Assessment: Insights into substrate binding and utilization by hyaluronan synthase

This study addresses a fundamental question in glycobiology by elucidating how a single-site processive enzyme orchestrates the alternating addition of sugars to synthesize complex polysaccharides such as hyaluronan.The findings are compelling, providing a clear mechanistic framework supported by strong experimental validation.Major strengths include the integration of high-resolution structural data with rigorous biochemical analyses, resulting in a well-supported model of hyaluronan assembly.