Reviewer #2 (Public Review): Rice stripe virus utilizes a Laodelphax striatellus salivary carbonic anhydrase to facilitate plant infection by direct molecular interaction

This valuable study presents a well-designed set of experiments demonstrating how a planthopper salivary carbonic anhydrase can promote rice stripe virus infection by modulating callose deposition in the host plant.The authors provide solid data for the proposed protein-protein interactions, including strengthened evidence for the LssaCA-NP-OsTLP complex and clarified dynamics of LssaCA presence in planta.Overall, the work reveals a mechanistic link whereby a vector salivary protein enhances a plant β-1,3-glucanase to suppress callose-based defense, thereby facilitating early viral establishment.