Reviewer #2 (Public review): ATP burst is the dominant driver of antibiotic lethality in Mycobacterium smegmatis

In this important work, Lodhiya et al. provide evidence that excessive ATP underlies the killing of the model organism Mycobacterium smegmatis by two mechanistically-distinct antibiotics.The data are generally solid as the authors deploy multiple, orthogonal readouts and methods for manipulating reactive oxygen species and ATP.The work will be of interest to those studying antibiotic mechanisms of action.