Effects of Isulsongi mushroom (Lentinula edodes GNA01) supplementation level and feeding period on serum lipid profiles in dogs

This study examined how the feeding level and duration of Isulsongi mushroom (Lentinula edodes GNA01) affected blood lipid profiles in dogs.Eighteen Maltese dogs were split into three groups: control (no supplement), 0.01% (100 mg•kg -1 ), and 0.02% (200 mg•kg -1 ).The mushroom powder was given by mouth for nine weeks.Blood was collected at 0, 3, 6, and 9 weeks to measure total cholesterol (TC), triglycerides (TGs), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C).From these data, atherogenic index (AI) and cardiac risk factor (CRF) were calculated.Data were analyzed by feeding level, feeding period, and their interaction.Dogs supplemented with the mushroom showed lower TC, TGs, and LDL-C and higher HDL-C than the control group.The 0.02% group showed the lowest TC and LDL-C and the highest HDL-C (p < 0.05).AI and CRF decreased as the supplementation level and time increased.These results indicate that Isulsongi mushroom helps regulate lipid levels and could be used as a functional ingredient for dogs.