Long-term, high-throughput time-lapse microscopy of mouse stem cells

Long-term, high-throughput time-lapse microscopy is a powerful tool for studying the differentiation processes of single cells in unprecedented temporal resolution. A high frequency of bright-field imaging (typically on the scale of a few minutes) ensures that moving single cells and cell divisions can be accurately tracked and used for the construction of cellular genealogies. Additionally, fluorescence imaging (which, owing to cell phototoxicity, is typically only possible when spaced out by intervals of 30 min or more) allows the quantification of molecular lineage markers. We here provide parts of the data used in Buggenthin et al., Nature Methods 2017, http://dx.doi.org/10.1038/nmeth.4182.