INDUKSI KALUS TANAMAN BUAH SALJU (Inga edulis Mart.) DENGAN PENAMBAHAN 2,4-DICHLOROPHENOXYACETIC ACID (2,4-D) SECARA IN VITRO

Inga edulis is a plant species belonging to the family Fabaceae that produces edible fruits with white, cotton-like pulp. Inga edulis is locally known as the snow fruit plant. Propagation of Inga edulis is commonly carried out using seeds due to their high germination success. However, this species has recalcitrant seed characteristics, making the seeds unsuitable for long-term storage because of rapid loss of viability. In vitro propagation can serve as an alternative solution to this problem. This research aimed to determine the optimal concentration of 2,4-D for callus growth from leaf and node explants of Inga edulis. The research was conducted from February to December 2025 at the Embryology Laboratory of UIN Sunan Kalijaga. The research design used RAL experimental was employed using leaf and node explants treated with 2,4-D at concentrations of 0, 1,5; 2; 2,5; 3,5; and 4.5 ppm, with five replications per treatment. The fastest callus initiation occurred at 14 days after planting (DAP), observed at 0 ppm in node explants and 2.5 ppm in leaf explants. Callus derived from leaf explants exhibited white, brownish-white, and brown coloration with friable and compact textures, whereas callus from node explants showed white, yellowish-white, and yellowish-green coloration with similar textures. The highest percentage of callus formation in both explant types was recorded at 4.5 ppm, reaching 25% in leaf explants and 16.66% in node explants. These results indicate that the application of 2,4-D significantly influences callus formation in leaf and node explants of Inga edulis.