Uspešno čiščenje DNK iz agaroznih čepkov PFGE za sekvenciranje celotnega genoma

Repository of the University of Ljubljana (University of Ljubljana) 2026 Open access

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Authors: Katja Strašek Smrdel, Andraž Celar Šturm, Tina Triglav, Mateja Pirš
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Abstract

Whole-genome sequencing (WGS) has replaced Pulsed-field gel electrophoresis (PFGE)-based bacterial genotyping as the reference genotyping method. We investigated the suitability of purified genomic DNA extracted from PFGE agarose plugs stored in a laboratory collection for WGS in cases where bacterial isolates are no longer available. Our study has shown that bacterial WGS can be successfully performed on DNA extracted from PFGE agarose plugs.

Fields of Study & Subjects

Genomics and Phylogenetic Studies Bacterial Identification and Susceptibility Testing Antimicrobial Resistance in Staphylococcus

Publication details

Journal / source
Repository of the University of Ljubljana (University of Ljubljana)
Publication date
12 February 2026
Language
EN