Therapeutic Efficacy of Ocimum sanctum L. and Anti-mastitis Herbal Paste on Subclinical Mastitis in Cow in Different Housing Systems

Background and Aims: Mastitis, mammary gland inflammation, is one of the most important diseases affecting dairy cattle globally, causing significant losses to farmers. Therefore, preventive techniques, including managemental practices, need to be applied to reduce mastitis. The current study was conducted to assess prophylactic potential of O. sanctum and anti-mastitis herbal paste in subclinical mastitis in cow. Methodology: Twenty-four HF crossbred cows diagnosed with subclinical mastitis were selected for therapeutic evaluation, while twelve healthy cows served as controls. The affected cows were randomly allocated into four treatment groups (n=6) based on housing system. Groups I (conventional housing) and II (loose housing) received Ocimum sanctum leaf powder orally, twice daily for five consecutive days. Similarly, Groups III (conventional housing) and IV (loose housing) were treated topically with an anti-mastitis paste containing Aloe vera, Curcuma longa, and lime, applied twice daily for five days. Results: Somatic cell count (SCC) values of infected quarters showed a marked decline (P