Characterization Of Ligand Binding To A Membrane Protein By On-Cell NMR Spectroscopy

Florence Research (University of Florence) 2026 Open access

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Authors: Love Elizabeth Afolayan, Enrico Luchinat, Letizia Berbieri, Lucia Banci
Cited by: 0

Abstract

We applied ligand-observed NMR strategies to investigate ligand binding to the membrane protein carbonic anhydrase IX (CA IX) in intact human cells. Using an on-cell NMR approach helps overcome the challenges associated with the isolation and purification of membrane proteins.

Fields of Study & Subjects

Enzyme function and inhibition Advanced NMR Techniques and Applications Lipid Membrane Structure and Behavior

Publication details

Journal / source
Florence Research (University of Florence)
Publication date
1 January 2026
Language
EN