Phytoremediation of anthelmintic-contaminated soil using energy crops

Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Ludmila Veselá Supervisor: prof. Ing. Barbora Szotáková, Ph.D. Consultant: RNDr. Radka Podlipná, Ph.D. Title of Diploma Thesis: Phytoremediation of anthelmintic-contaminated soil using energy crops Anthelmintics are veterinary drugs used against parasitic worms. They contaminate the environment they enter during prophylactic treatment of animals, both on pastures, where drug residues are directly excreted into the environment, and using manure from treated animals. This thesis focuses on the ability of maize (Zea mays) to uptake selected anthelmintics (albendazole, ricobendazole, and ivermectin) from soil, while also monitoring the effects of these substances on the composition of soil prokaryotic communities. Plants were grown in soil contaminated with individual anthelmintics at two concentrations, and sampling was carried out at two time intervals. To determine drug concentrations in roots and soil, HPLC was used for high concentrations, and UHPLC-MS for low concentrations and root samples. The composition of the microbial community was analyzed by sequencing the V4 variable region of the 16S rRNA gene. The results showed that maize can absorb and accumulate anthelmintics in the roots, and the...