Electrochemical Determination of Genotoxic Substances

Ph.D. thesis is focused on development of methods for voltammetric and amperometric determination of trace amounts of genotoxic nitroquinolines (NQs) and aminoquinolines (AQs) utilizing non-toxic alternative electrodes, silver solid amalgam electrode (AgSAE) and carbon film electrode (CFE). Some properties of given electrodes and associated materials as well as electrochemical behaviour of listed substances were investigated, too. Comparison study of amalgam-based materials with liquid mercury films and dental amalgam in terms of mercury vapour pressure was carried out by AAS method of cold vapours. Further, influence of carbon concentration in the CFE's film on detection properties and the size of CFE's potential window in different media was investigated and compared to selected SPEs. Moreover, CFE's repeatability and reproducibility study was carried out. For electrochemical analysis of 5-nitroquinoline (5-NQ) and 6-nitroquinoline (6-NQ), mercury meniscus modified AgSAE was used. Electrochemical behaviour of both NQs was characterized using CV and DPV. Determination of NQs was carried out by DPV and DCV, of 5-NQ also by FIA with electrochemical detection (FIA-ED), by which determination was possible also in drinking and river water. Limits of quantitation (LOQs) achieved under optimized...