Intestinal Stem Cells: Methods and Protocols (Methods in Molecular Biology): Primary cultures of foetal epithelial progenitors
Abstract
In this chapter, we present a protocol for isolating fetal epithelial progenitors from the murinesmall intestine and colon, and for culturing them ex vivo using the three-dimensional organoidsystem. We also describe cellular approaches to characterize Lgr5-negative fetal progenitorsand compare their identity and differentiation potential to those of Lgr5-positive adult intestinalstem cells. This system provides a valuable tool for studying the biology of fetal progenitorsand exploring the developmental origins of adult intestinal stem cells.
Fields of Study & Subjects
Cancer Cells and Metastasis
Congenital gastrointestinal and neural anomalies
Renal and related cancers
Publication details
- Journal / source
- Dépôt institutionnel de l'Université libre de Bruxelles (Université Libre de Bruxelles)
- Publication date
- 1 February 2026
- Language
- EN
- Document type
- Book chapter