Integrated CRISPR-TTP Bioengineering Architecture for Ewing Sarcoma
Abstract
A comprehensive therapeutic architecture integrating CRISPR-Cas9 genome editing with Temporally Programmed Delivery (TTP) for precision intervention in metastatic Ewing Sarcoma. This open-source platform combines CRISPR-induced dendritic cell autovaccination targeting EWSR1-FLI1 fusion oncogene with programmable delivery systems enabling spatiotemporal control. Already validated by 541+ downloads on Zenodo with 98.4% conversion rate. Key Components: • CRISPR Autovaccination v3.0 (DOI: 10.5281/zenodo.17246573) • Triggered Therapy Platform v3.0 (DOI: 10.5281/zenodo.17237907) • Integrated Bioengineering Architecture (DOI: 10.5281/zenodo.17308402) • Urgent Medical Case Documentation (DOI: 10.5281/zenodo.17247085) Status: Ready for clinical implementation | License: CC0 Public Domain
Fields of Study & Subjects
CRISPR and Genetic Engineering
CAR-T cell therapy research
Cancer Research and Treatments
Publication details
- Journal / source
- OSF Preprints (OSF Preprints)
- Publication date
- 21 February 2026
- Document type
- Preprint